Scientists transformed energy-storing white fat cells into calorie-burning ‘beige’ fat. Once implanted, they outcompeted tumors for resources, beating back five different types of cancer in lab experiments.
Scientists at UC San Francisco have pioneered a groundbreaking approach to fighting cancer using engineered fat cells.
By transforming ordinary fat into high-energy beige fat using CRISPR, they created a cell type that voraciously consumes nutrients — outcompeting and starving tumors.
Revolutionizing Cancer Treatment: Engineered Fat Cells Starve Tumors
Liposuction and plastic surgery might seem unrelated to cancer treatment, but they’ve inspired a groundbreaking new approach.
Researchers at UC San Francisco have developed a technique that uses engineered fat cells to starve tumors. Using CRISPR gene-editing technology, they transformed ordinary white fat cells into “beige” fat cells — cells that burn calories aggressively to generate heat.
To test their effectiveness, scientists implanted these modified fat cells near tumors, much like how plastic surgeons transfer fat from one area of the body to another. The result? The engineered fat cells rapidly consumed nutrients, depriving tumor cells of the fuel they need to survive. Remarkably, this approach worked even when the fat cells were placed far from the tumor site in mice.
Because the method builds on existing medical procedures, it could pave the way for a faster transition to clinical use as a promising new cancer therapy.
“We already routinely remove fat cells with liposuction and put them back via plastic surgery,” said Nadav Ahituv, PhD, director of the UCSF Institute for Human Genetics and professor in the Department of Bioengineering and Therapeutic Sciences. He is the senior author of the paper, which appears Feb. 4 in Nature Biotechnology.
“These fat cells can be easily manipulated in the lab and safely placed back into the body, making them an attractive platform for cellular therapy, including for cancer.”
Beige Fat Cells Outcompete Cancer for Nutrients
Ahituv and his post-doc at the time, Hai Nguyen, PhD, were aware of studies that showed exposure to cold could suppress cancer in mice.
One remarkable experiment even showed it could help a patient with non-Hodgkin lymphoma. Scientists concluded that the cancer cells were starving because the cold was activating brown fat cells, which use nutrients to produce heat.
But cold therapy isn’t a viable option for cancer patients with fragile health.
So, Ahituv and Nguyen turned to the idea of using beige fat, wagering that they could engineer it to burn enough calories, even in the absence of cold, to deprive tumors of the fuel they needed to grow.
CRISPR Unlocks the Power of Beige Fat
Nguyen, who is the first author of the paper, used CRISPR to activate genes that are dormant in white fat cells but are active in brown fat cells, in the hopes of finding the ones that would transform the white fat cells into the hungriest of beige fat cells.
A gene called UCP1 rose to the top.
Then, Nguyen grew UCP1 beige fat cells and cancer cells in a “trans-well” petri dish. The cancer cells were on the bottom and the fat cells were above them in separate compartments that kept the cells apart but forced them to share nutrients.
The results were shocking.
“In our very first trans-well experiment, very few cancer cells survived. We thought we had messed something up – we were sure it was a mistake,” Ahituv recalled. “So, we repeated it multiple times, and we kept seeing the same effect.”
The beige fat cells held sway over two different types of breast cancer cells, as well as colon, pancreatic, and prostate cancer cells.
But the researchers still didn’t know if the implanted beige fat cells would work in a more realistic context.
Fat Cell Therapy Proves Its Power Against Multiple Cancers
So, the scientists turned to fat organoids, which are coherent clumps of cells grown in a dish, to see if they could beat tumor cells when they were implanted next to tumors in mice.
The approach worked against breast cancer, as well as pancreatic and prostate cancer cells. The cancer cells starved as the fat cells gobbled up all the available nutrients.
The implanted beige fat cells were so powerful that they suppressed pancreatic and breast tumors in mice that were genetically predisposed to develop cancer. It even worked when the beige fat cells were implanted far away from the breast cancer cells.
To see how they would work in human tissue, Ahituv and Nguyen teamed up with Jennifer Rosenbluth, MD, PhD, a breast cancer specialist at UCSF. Rosenbluth had amassed a library of breast cancer mastectomies containing both fat cells and cancer cells.
“Because the breast has a lot of fat, we could get fat from the same patient, modify the fat, and grow it in a single trans-well experiment with that patient’s own breast cancer cells,” Ahituv said.
These same-patient beige fat cells outcompeted breast cancer cells in petri dishes – and when they were implanted together in mouse models.
Knowing that cancers have preferred diets, the researchers engineered fat just to eat certain nutrients. Certain forms of pancreatic cancer, for example, rely on uridine when glucose is scarce.
So, they programmed the fat to eat just uridine, and they easily outcompeted these pancreatic cancer cells. This suggests that fat could be adapted to any cancer’s dietary preferences.
Fat Cells: The Future of Living Cell Therapy?
Fat cells have many advantages when it comes to living cell therapies, according to Ahituv.
Fat cells are easy to obtain from patients. They grow well in the laboratory and can be engineered to express different genes and take on different biological roles. And they behave well once they are put back into the body, not straying from the location where they’re implanted and playing nice with the immune system.
It’s a record borne out by decades of progress in plastic surgery.
“With fat cells, there’s less interaction with the environment, so there’s very little worry of the cells leaking out into the body, where they might cause problems,” Ahituv said.
Fat cells can also be programmed to emit signals or carry out more complicated tasks.
And their ability to defeat cancer even when they are not right next to tumors could prove invaluable for treating hard-to-reach cancers like glioblastoma, which affects the brain, as well as many other diseases.
Beyond Cancer: The Limitless Potential of Engineered Fat
“We think these cells could also be designed to sense glucose in the bloodstream and release insulin, for diabetes, or suck up iron in diseases where there’s excessive iron, like hemochromatosis,” Ahituv said. “The sky’s the limit for these fat cells.”
Reference: 4 February 2025, Nature Biotechnology.
DOI: 10.1038/s41587-024-02551-2
Authors: In addition to Ahituv, Nguyen, and Rosenbluth, other UCSF authors include Kelly An, Yusuke Ito, Bhushan N. Kharbikar, PhD, Rory Sheng, Breanna Paredes, MS, Elizabeth Murray, Kimberly Pham, Michael Bruck, Xujia Zhou, MS, PhD, Cassidy Biellak, Aki Ushiki, PhD, Mai Nobuhara, MS, Daniel A. Bernards, PhD, Mark Jesus M. Magbanua, PhD, Laura A. Huppert, MD, Heinz Hammerlindl, PhD, Laura Esserman, MD, MBA, Tejal A. Desai, PhD, and Sook Wah Yee, MPharm. For all authors see the paper.
Funding: The work was funded in part by the UCSF Sandler Program for Breakthrough Biomedical Research, the UCSF Living Therapeutics Initiative, the National Institutes of Health (1R01DK124769, 1R01CA283826) and the California Institute for Regenerative Medicine. Ahituv is a cofounder and on the scientific advisory board of Regel Therapeutics.
Disclosures: Ahituv receives funding from BioMarin Pharmaceutical Incorporate. Ahituv has filed a patent application covering embodiments and concepts disclosed in the manuscript. For all funding and disclosures see the paper.
